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[ 特殊顯微鏡設備 | TurboScan自動化高速掃描玻片影像系統 ]

■ TurboScan 自動化高速掃描玻片影像系統

Objective Imaging 的自動化高速掃描玻片影像系統( Surveyor Automated Scanning and Imaging with Turboscan ),利用拍照設備最快的速度,進行影像快速掃描拍照程序 。

快速的掃描拍照整個玻片,讓玻片內的細胞組織等檢測目標,快速的呈現出完整樣貌,讓研究者可以在很短的時間內,了解整個細胞組織的狀況,以便與顯微觀察的檢測關係相結合。

TurboScan只需簡單的滑鼠點擊,便可以檢測任何想要觀察或確認的玻片位置,對於研究者後續重覆檢測確認、研討、發佈等工作,都可以節省很多的時間。

基於 OASIS Automation Controller 與特殊的相機觸發功能,TurboScan提供一套理想的自動化掃片解決方案,包含樣品的定位、掃描、隨機拍照、自動化拍照存檔等等。

結合 Prior PL200 玻片自動載入機,可容納兩百片玻片,更可以達成大量的自動化玻片掃描工作,適用於大量病理切片顯微影像掃描應用,對於有大量玻片顯微拍照的工作者,是最佳的選擇。

相關應用:玻片掃描、病理切片掃描

自動化高速掃描玻片影像系統

■ 範例影像

Mouse
Zebra Fish
Appendix
Eye
Mouse Foetus
Eye
Skin
Mouse, Transverse Section
10X Objective / 1X C-mount
Calibration: 0.46 µm/pixel
Scan fields: 17 x 20, 340 total
Size at full resolution: 340 x 4.2 MB = 1.4 GB
Scan area: 10.6 x 9.2 mm = 98 sq. mm
Scan time (snake scan with predictive focus and shading correction): 42 secs
Mouse Transverse Section
Overall Structure
Mouse Transverse Section
Detail
Mouse Transverse Section
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Zebra Fish
10X Objective / 1X C-mount
Calibration: 0.46 µm/pixel
Scan fields: 16 x 17, 272 total
Size at full resolution: 272 x 4.2 MB = 1.2 GB
Scan area: 9.2 x 7.3 mm = 67 sq. mm
Scan time (snake scan with predictive focus and shading correction): 37 secs

Zebra Fish
Overall Structure
Zebra Fish
Detail
Zebra Fish
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Appendix, Human
10X Objective / 1X C-mount
Calibration: 0.46 µm/pixel
Scan fields: 16 x 18, 288 total
Size at full resolution: 288 x 4.2 MB = 1.25 GB
Scan area: 10.1 x 8.2 mm = 83 sq. mm
Scan time (raster scan with predictive focus and shading correction): 54 secs

Appendix
Overall Structure
Appendix
Detail
Appendix
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Mouse Foetus, Lateral Section
10X Objective / 1X C-mount
Calibration: 0.46 µm/pixel
Scan fields: 20 x 16, 320 total
Size at full resolution: 320 x 4.2 MB = 1.3 GB
Scan area: 12.5 x 7.4 mm = 93 sq. mm
Scan time (snake scan with predictive focus and shading correction): 46 secs

Mouse Foetus
Overall Structure
Mouse Foetus
Detail
Mouse Foetus
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Eye Section, Rabbit
10X Objective / 1X C-mount
Calibration: 0.46 µm/pixel
Scan fields: 19 x 23, 437 total
Size at full resolution: 437 x 4.2 MB = 1.8 GB
Scan area: 11.9 x 10.6 mm = 126 sq. mm
Scan time (raster scan with predictive focus and shading correction): 1 min 19 secs


Overall Structure
Mouse Foetus
Detail
Mouse Foetus
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Human Skin, Showing Follicles
10X Objective / 1X C-mount
Calibration: 0.46 µm/pixel
Scan fields: 15 x 15, 225 total
Size at full resolution: 225 x 4.2 MB = 0.95 GB
Scan area: 9.4 x 6.9 mm = 65 sq. mm
Scan time (raster scan with predictive focus and shading correction): 38 secs

Human Skin
Overall Structure
Human Skin
Detail
Human Skin
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■ 功能特點

Mosaic Acquisition

  • Turboscan for fast continuous scan and acquire
    Standard scan for step and acquire for low light
    applications
  • Snake and raster scanning
  • User-defined settle time at each field
  • Pause and continue scanning mode
  • Grab individual fields into mosaic with key-press
  • Acquire at full or reduced resolution
  • JPEG compression option
  • Image streaming for mosaic sizes limited only by
    free disk space

Specimen Map

  • Stage and pattern views
  • Point and click relocation to position or pattern field
  • Real-time graphic display of current stage position
    on specimen map
  • Zoom and pan
  • Drag-drop pattern definition
  • Acquire and display multiple mosaics at different
    magnifications

Scan Patterns

  • Rectangle
  • Circular
  • Annular
  • Cross ("+", "x")
  • Random
  • User-defined
  • Save / load scan patterns
  • Keyboard support for pattern scanning and stage
    navigation

Calibration

  • X and Y calibration factors
  • Stored calibration table for multiple lens and
    secondary optic settings
  • Guided camera to stage alignment procedure
  • Automatic correlation-based calibration procedure
  • Automatic stage orthogonality correction

Camera Support

  • Image setup based on camera capabilities
  • Exposure, gain, offset, and gamma control as
    available
  • Automatic and manual white balance
  • Color or monochrome acquisition
  • Frame-rate shading correction
  • Shading correction on live display
  • Calibrated graticule overlay on live image
  • Grab, copy, save live image

Microscope Automation

  • OASIS-4i XY stage and Z focus
  • Software joystick for stage, focus, turret, and
    lamp control
  • Leica Microsystems DM range focus, turret,
    condenser, and lamp control via RS-232
  • Olympus BX-61 focus, turret, condenser, and
    lamp control via RS-232
  • Stage and focus speed defined per objective
  • Computer controlled parfocality and illumination
    defined per objective

Export / Import

  • Workspace save and load of full mosaic, scan
    pattern and Surveyor envirnoment
  • Export mosaic as full resolution bitmap
  • Export mosaic as reduced resolution bitmaps
  • Export user-selected region of interest
  • Print full mosaic or selected region
  • Print preview

Automatic Focus

  • Multi-point predictive focus for continuous focus
    tracking
  • Predictive focus setup wizard
  • Video autofocus using OASIS-AF module
  • Digital camera autofocus using OASIS-DC1 module
  • Autofocus parameters stored separately for each
    objective lens
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